Development of SSR Markers Associated with Polima CMS Fertility Restorer in <i>Brassica napus</i> L. and Its Application

The Polima system of cytoplasmic male sterility (Pol CMS) was widely used for hybrid seed production, but with high probability occurrence maternal false due to the fertility CMS line greatly affected by ambient temperature, therefore, detection hybrids is key purity identification. In present study, SSR markers were designed based on flanking sequences Pol restorer gene&nbsp; Rfp , and application in test rapeseed. main results as follows: &nbsp;Rfp&nbsp; subjected basic local alignment search tool queries against the&nbsp; Brassica rapa &nbsp;genome determine chromosome positions, 32 developed upstream downstream of&nbsp; . Among markers, one codominant marker polymorphic between sterile line, PCR product length 162 bp lines, 184 lines. Based sequence, two insert fragments (15 7 bp) identified co-segregated plant individuals &ldquo;fengyou737&rdquo; F 2 &nbsp;population. &ldquo;fengyou737&rdquo;, &ldquo;fengyou320&rdquo; &ldquo;fengyou958&rdquo; means PCR-SSR field-planting, ways highly consistent, indicated that tightly linked to&nbsp; can detect paternal hybrids. findings will provide technical assistance genetic diversity analysis